Here are the magnifications and corresponding pixel sizes for the UltraScan 4000 CCD camera (as determined using serialEM and a replica diffraction grating). The values shown in blue correspond to the Low Mag mode of operation while the values in black correspond to the normal Mag mode.

 

Calibrated Pixel Size (TEM Mode)
 
 
magnification
pixel size (nm)
 
 
120
90.65
150
74.90
200
53.29
250
44.22
300
36.94
400
28.50
500
23.08
600
19.41
800
14.65
1000
11.83
1200
9.74
1500
7.77
2000
5.65
2500
4.82
3000
3.94
2500
4.93
3000
3.90
4000
2.65
5000
2.19
6000
1.84
8000
1.45
10000
1.15
12000
0.93
15000
0.74
20000
0.56
25000
0.45
30000
0.38
40000
0.30
50000
0.21
60000
0.18
80000
0.15
100000
0.12
120000
0.11

Higher magnifications should be calibrated using the lattice spacing of gold or graphite on the day of use of the JEOL JEM 3200FS.

NOTE: The pixel size shown in this chart and that of Digital Micrograph will often differ slightly. This is a consequence of differences in the way the pixel size was determined and whether the pixel size is a direct measurement or an interpoated value. Also, the 3200FS shows significant defocus-dependent magnification changes starting around 2 micrometers from focus and the defocus of specific images will definitely affect the exact magnification of the recorded image..