pEarleyGate Vectors -features, maps and ordering information
In the table below, clicking on the ABRC stock number should take you directly to the ABRC webpage where you can order that clone. Should a hyperlink not work, please report the dead link to Craig Pikaard so that it can be repaired. In the meantime, you can search ABRC for pEarleyGate vectors by going to the TAIR website, finding the "Stocks" sub-heading and choosing "Search DNA stocks" using"vector" as the "search for" term.
For those researchers using Gene Construction Kit software (http://www.textco.com), GCK files of each sequence are included as links in the table below. To obtain the GCK file, Mac users may need to click the mouse while pressing the option key to download the file or may need to hold down the mouse and choose the save to disk option.
Earley, Keith, Jeremy R. Haag, Olga Pontes, Kristen Opper,
Tom Juehne, Keming Song, and Craig S. Pikaard (2006). Gateway-compatible
vectors for plant functional genomics and proteomics. The Plant
J. 45:616-629. See
abstract at Pubmed.
| Plasmid name | Description | Circular map (+ unique sites) |
|
plasmid sequence (Gene Construction Kit) |
|
| pEarleyGate 100 | 35S-Gateway-OCS 3' | image file | text file | GCK file | CD3-724 |
| pEarleyGate 101 | 35S-Gateway-YFP-HA tag-OCS 3' | image file | text file | GCK file | CD3-683 |
| pEarleyGate 102 | 35S-Gateway-CFP-HA tag-OCS 3' | image file | text file | GCK file | CD3-684 |
| pEarleyGate 103 | 35S-Gateway-GFP-His tag-OCS 3' | image file | text file | GCK file | CD3-685 |
| pEarleyGate 104 | 35S-YFP-Gateway-OCS 3' | image file | text file | GCK file | CD3-686 |
| pEarleyGate 201 | 35S-HA tag-Gateway-OCS 3' | image file | text file | GCK file | CD3-687 |
| pEarleyGate 202 | 35S-FLAG tag-Gateway-OCS 3' | image file | text file | GCK file | CD3-688 |
| pEarleyGate 203 | 35S-Myc tag-Gateway-OCS 3' | image file | text file | GCK file | CD3-689 |
| pEarleyGate 204 | 35S-AcV5 tag-Gateway-OCS 3' | image file | text file | GCK file | CD3-690 |
| pEarleyGate 205 | 35S-Gateway-TAP tag-OCS 3' | image file | text file | GCK file | CD3-691 |
| pEarleyGate 301 | no promoter-Gateway-HA tag-OCS3' | image file | text file | GCK file | CD3-692 |
| pEarleyGate 302 | no promoter-Gateway-FLAG tag-OCS3' | image file | text file | GCK file | CD3-693 |
| pEarleyGate 303 | no promoter-Gateway-Myc tag-OCS3' | image file | text file | GCK file | CD3-694 |
| pEarleyGate 304 | no promoter-Gateway-AcV5 tag-OCS3' | image file | text file | GCK file | CD3-695 |